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Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
This content is Free Access.
Chapters
- 00:04Introduction
- 00:52Sample Preparation
- 01:58Lightsheet Microscope Setup
- 04:36Image Analysis
- 06:23Results: Time-Lapse Imaging of Early Eye Development in Zebrafish
- 07:17Conclusion
Here, a protocol is provided for time-lapse imaging of ocular morphogenesis using a commercially available lightsheet microscope and an image processing workstation to analyze the resulting data. This protocol details the procedures for embryo anesthesia, embedding in low melting temperature agarose, suspension in the imaging chamber, setting up the imaging parameters, and finally analyzing the imaging data using image analysis software.
