Journal
/
Developmental Biology
/
A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

DOI:
10.3791/62318-v

•

08:06 min

•

March 19, 2021

•

, ,
1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht

Chapters

  • 00:04Introduction
  • 00:50Generation of the Chip
  • 02:21Preparing Microfluidics Experiment
  • 03:44Microfluidics Experiment
  • 05:57Results: Entrainment Analysis Using Microfluidics Experiments
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis.

Tags

Keywords: MicrofluidicsSignaling OscillationsSomitogenesisSignaling DynamicsPDMSPlasma BondingFibronectin CoatingMouse EmbryosFunctional Dissection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62318?article_type=v

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
check_url/62318?article_type=v

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System
check_url/62318?article_type=v

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
check_url/62318?article_type=v

Functional Cloning Using a Xenopus Oocyte Expression System
check_url/62318?article_type=v

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
check_url/62318?article_type=v

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
check_url/62318?article_type=v

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
check_url/62318?article_type=v

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
check_url/62318?article_type=v

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
check_url/62318?article_type=v

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Read Article