Journal
/
Developmental Biology
/
Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

DOI:
10.3791/55035-v

•

09:32 min

•

January 08, 2017

•

, ,
1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine,Yeungnam University, 2Physiology and Experimental Medicine Program,The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology,University of Toronto

Chapters

  • 00:05Title
  • 00:49Mouse Embryonic Stem Cell (mESC) Culture
  • 02:06Serum- and Feeder-free Conditioned Medium Collection
  • 03:17Senescence-associated (SA) Beta Galactosidase Assay
  • 04:46Cell Cycle and qRT-PCR Analyses
  • 07:19Results: Characterization of the Effects of mESC-conditioned Medium on Cell Growth, Cycle Stage, and Function
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol provides a method for the collection of mouse embryonic stem cell (mESC)-conditioned medium (mESC-CM) derived from serum (fetal bovine serum, FBS)- and feeder (mouse embryonic fibroblasts, MEFs)-free conditions for a cell-free approach. It may be applicable for the treatment of aging and aging-associated diseases.

Tags

Keywords: Mouse Embryonic Stem CellsSerum-freeFeeder-freeConditioned MediumCell-freeAnti-senescenceCell CultureFibroblastsTrypsinGelatinReduced Serum MediumSenescence-associated Beta-galactosidase

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55035?article_type=v

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
check_url/55035?article_type=v

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
check_url/55035?article_type=v

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
check_url/55035?article_type=v

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
check_url/55035?article_type=v

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
check_url/55035?article_type=v

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
check_url/55035?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/55035?article_type=v

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
check_url/55035?article_type=v

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
check_url/55035?article_type=v

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Read Article