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Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions
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JoVE Journal Developmental Biology
Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

DOI:
10.3791/60843-v

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10:24 min

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May 24, 2020

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1Center for Molecular Medicine,University of Georgia, 2Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences,University of Georgia, 3Department of Cellular Biology, Franklin College of Arts and Sciences,University of Georgia

Chapters

  • 00:04Introduction
  • 00:50Human Pluripotent Stem Cell (hPSC) Maintenance
  • 01:48hPSC Differentiation (Day 0)
  • 03:34Neural Crest Cell (NCC) Induction (Day 1-10)
  • 04:02NC Spheroid Aggregation (Day 10)
  • 05:14NC Spheroid Maintenance and Sympathetic Progenitor Induction (Day 11-14)
  • 06:09Sympathetic Neuron (symN) Differentiation and Maturation (Day 14-20 or 28-35)
  • 07:38Results: Representative NC Induction, Maintenance, and Expansion
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, we describe a stable, highly efficient differentiation strategy for the generation of postganglionic sympathetic neurons from human pluripotent stem cells. This model will make neurons available for the use of studies of multiple autonomic disorders.

Tags

Human Pluripotent Stem CellsSympathetic NeuronsFeeder-freeChemically DefinedEfficient DifferentiationElectrical ActivityDisease ModelingRegenerative MedicineDrug ScreeningCo-culture System

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