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JoVE Journal
Immunology and Infection

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Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
 

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Article DOI: 10.3791/62368-v 07:42 min April 16th, 2021
April 16th, 2021

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Summary

A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.

Tags

Keywords: Mycobacteria Lipids Thin Layer Chromatography Extraction Chloroform Methanol Mycolic Acid N-hexane TLC
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