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Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
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Immunology and Infection
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JoVE Journal Immunology and Infection
Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

DOI:
10.3791/62437-v

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09:45 min

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May 18, 2021

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, , ,
1College of Veterinary Medicine (Institute of comparative medicine),Yangzhou University, 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 3Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China,Yangzhou University

Chapters

  • 00:04Introduction
  • 01:04Hybridoma Technology to Produce Monoclonal Antibodies (mAbs) Against Aminopeptidase N (APN)
  • 04:20Expression of Recombinant Antibodies (rAbs) Against APN
  • 06:25Results: Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

The recombinant antibody protein expressed in pIRES2-ZSGreen1-rAbs-APN-CHO cells and monoclonal antibodies produced using traditional hybridoma technology can recognize and bind to the porcine aminopeptidase N (APN) protein.

Tags

Keywords: Monoclonal AntibodiesAminopeptidase NPorcine Intestinal Mucosal EpitheliumRecombinant Antibody ProductionAntibody Drug ConjugatesHybridizationSP20 Myeloma CellsPeritoneal MacrophagesPolyethylene Glycol

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