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猪肠粘膜上皮中靶向氨基肽酶N的单克隆抗体的生产
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
DOI:

09:45 min

May 18, 2021

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Chapters

  • 00:04Introduction
  • 01:04Hybridoma Technology to Produce Monoclonal Antibodies (mAbs) Against Aminopeptidase N (APN)
  • 04:20Expression of Recombinant Antibodies (rAbs) Against APN
  • 06:25Results: Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
  • 09:13Conclusion

Summary

Automatic Translation

pIRES2-ZSGreen1-rAbs-APN-CHO细胞表达的重组抗体蛋白和采用传统杂交瘤技术生产的单克隆抗体可以识别并与猪氨基肽酶N(APN)蛋白结合。

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