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Journal
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Biochemistry
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格勒诺布尔EMBL HTX工厂的自动化晶体学管道
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
Please note that all translations are automatically generated.
Click here for the English version.
格勒诺布尔EMBL HTX工厂的自动化晶体学管道
DOI:
10.3791/62491-v
•
06:50 min
•
June 05, 2021
•
Irina Cornaciu
2
,
Raphael Bourgeas
,
Guillaume Hoffmann
,
Florine Dupeux
3
,
Anne-Sophie Humm
,
Vincent Mariaule
,
Andrea Pica
2
,
Damien Clavel
2
,
Gael Seroul
2
,
Peter Murphy
,
José Antonio Márquez
2
1
European Molecular Biology Laboratory
,
2
ALPX S.A.S.
,
3
Institut de Biologie Structurale
Chapters
00:04
Introduction
00:32
Crystallization Screening Experiments
02:42
Crystal Optimization Experiments
03:18
Crystal Harvesting and Tracking
04:21
Synchrotron X-Ray Diffraction Experiments
05:27
Results
06:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了如何使用自动化大分子晶体学管道进行蛋白质到结构,快速配体 - 蛋白质复合物分析和基于EMBL格勒诺布尔HTX实验室的CrystalDirect技术的大规模片段筛选。
Tags
Keywords: Automated Crystallography
EMBL HTX Facility
Grenoble
CrystalDirect Technology
CRIM Software
Crystal Graphic Information Management System
Crystallization Protocol
Crystallization Screens
High Throughput Crystallization Lab
Automated Imagers
CRIMS
Crystallization Plates
Crystallization Experiments
Crystal Harvesting
Protein Structure Determination
Fragment Screening
Ligand Screening
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