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格勒诺布尔EMBL HTX工厂的自动化晶体学管道
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
DOI:

06:50 min

June 05, 2021

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Chapters

  • 00:04Introduction
  • 00:32Crystallization Screening Experiments
  • 02:42Crystal Optimization Experiments
  • 03:18Crystal Harvesting and Tracking
  • 04:21Synchrotron X-Ray Diffraction Experiments
  • 05:27Results
  • 06:08Conclusion

Summary

Automatic Translation

在这里,我们描述了如何使用自动化大分子晶体学管道进行蛋白质到结构,快速配体 - 蛋白质复合物分析和基于EMBL格勒诺布尔HTX实验室的CrystalDirect技术的大规模片段筛选。

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