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マウスの新鮮な骨髄のプロ血小板形成ダイナミクス
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Biology
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JoVE Journal
Biology
Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants
Please note that all translations are automatically generated.
Click here for the English version.
マウスの新鮮な骨髄のプロ血小板形成ダイナミクス
DOI:
10.3791/62501-v
•
05:58 min
•
May 20, 2021
•
Inès Guinard
,
François Lanza
,
Christian Gachet
,
Catherine Léon
,
Anita Eckly
1
Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS
Chapters
00:04
Introduction
01:22
Preparation of the Experimental Set Up
01:51
Bone Marrow Sectioning and Placement into the Incubation Chamber
03:13
Real-Time Observation of Marrow Explants and Quantification of the Proplatelet-Extending Megakaryocytes
04:02
Results: Morphological Assessment of Megakaryocytes in Explants
04:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、骨髄外植法について詳述し、サンプル調製から顕微鏡スライド解析まで、それらの生理環境において分化した巨核球がプロ血小板を形成する能力を評価する。
Tags
Keywords: Proplatelet Formation
Mouse Bone Marrow Explants
Megakaryocytes
Real-time Analysis
Thrombophilic Disease
Tyrode’s Buffer
Incubation Chamber
Time-lapse Microscopy
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