Journal
/
Biology
/
在苹果纸浆中用 Fluo-4/AM 染色细胞质 Ca2+
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
Please note that all translations are automatically generated. Click here for the English version.

在苹果纸浆中用 Fluo-4/AM 染色细胞质 Ca2+

DOI:
10.3791/62526-v

•

08:05 min

•

November 06, 2021

•

, , ,
1College of Horticulture,Qingdao Agricultural University

Chapters

  • 00:03Introduction
  • 00:57Protoplast Extraction
  • 01:49Small-molecule Calcium Ion Fluorescence Staining
  • 04:46Protoplast Viability Assay
  • 05:48Results
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

苹果浆细胞的分离原体装有荧光钙试剂，以检测细胞质Ca2+ 浓度。

Tags

Cytoplasmic Ca2+Fluo-4/AMApple PulpCalcium ImagingProtoplast ExtractionSmall Molecule Fluorescent IndicatorCalcium Ion SelectivityEsterification IncubationEnzymatic SolutionFluorescence Microscope

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

焦距钙 2 +</sup瞬态检测平滑肌

在体内的Ca 2 + -蜜蜂的嗅觉学习期间的蘑菇体神经元的成像

技术成像钙 2 +信号在人类精子

活细胞钙成像相结合的siRNA介导的基因沉默标识CA 2 +泄漏通道内质网膜和监管机制

激活细胞凋亡的细胞色素的细胞质显微注射 C</em

可视化细胞质流动过程中单细胞伤口愈合<em>蓝斑宏声</em

动态钙的自动分析<sup> 2 +</sup>在图像序列信号

细胞质DNA的检测途径的刺激<em>在体外</em>和<em>体内</em

成像本地CA<sup> 2+</sup>在培养的哺乳动物细胞信号

将细胞质Foci分类为哺乳动物应激颗粒的方法

Read Article