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Färgning av cytoplasmic Ca2+ med Fluo-4/AM i Äppelmassa
JoVE Journal
Biology
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JoVE Journal Biology
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
DOI:

08:05 min

November 06, 2021

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Chapters

  • 00:03Introduction
  • 00:57Protoplast Extraction
  • 01:49Small-molecule Calcium Ion Fluorescence Staining
  • 04:46Protoplast Viability Assay
  • 05:48Results
  • 07:21Conclusion

Summary

Automatic Translation

Isolerade protoplaster av äppelmassa celler laddades med en kalcium fluorescerande reagens för att upptäcka cytoplasmic Ca2 + koncentration.

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