Journal
/
Neuroscience
/
Optagelse af fluorescerende mærket små ekstracellulære vesikler In Vitro og i rygmarven
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Optagelse af fluorescerende mærket små ekstracellulære vesikler In Vitro og i rygmarven

DOI:
10.3791/62537-v

•

09:01 min

•

May 23, 2021

•

, , , ,
1Department of Pharmacology & Physiology,Drexel University College of Medicine

Chapters

  • 00:04Introduction
  • 00:57Uptake of sEVs by Neuro-2a Cells
  • 01:38Primary Astrocytic Cultures
  • 03:34Immunofluorescence
  • 05:03In Vivo Uptake of sEVs and Immunohistochemistry
  • 06:43Results: Uptake of Fluorescent Labeled Small Extracellular Vesicles
  • 08:11Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en protokol til mærkning makrofag-afledte små ekstracellulære vesikler med PKH farvestoffer og observere deres optagelse in vitro og i rygmarven efter intrathecal levering.

Tags

Fluorescent Labeled Small Extracellular VesiclesSEVs UptakeSpinal CordIn VitroCell VisualizationNeuro-2a CellsPostnatal PupsCortical LobesDissociationAstrocytesMicrogliaOligodendrocytesMechanistic StudiesTherapeutic StudiesSpinal DisordersPainInflammation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62537?article_type=v

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
check_url/62537?article_type=v

Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication
check_url/62537?article_type=v

Retrograd fluorescensmærkning Tillader Målrettet Extracellular Single-unit Optagelse fra Identificerede Neuroner<em> In vivo</em
check_url/62537?article_type=v

Spinal Cord transection i larvestadiet zebrafisk
check_url/62537?article_type=v

En<em> Ex vivo</em> Laser-induceret Rygmarvsskader model til opgørelse Mekanismer axon Degeneration i realtid
check_url/62537?article_type=v

To-foton Imaging for Cellulær Dynamics i Mouse Spinal Cord
check_url/62537?article_type=v

Imaging neurale aktivitet i den primære somatosensoriske Cortex ved hjælp af Thy1-GCaMP6s Transgene mus
check_url/62537?article_type=v

En In Vivo Duo-farve metode for Imaging vaskulære Dynamics efter Contusive rygmarvsskade
check_url/62537?article_type=v

Laminektomi og rygmarv vindue implantation i musen
check_url/62537?article_type=v

Forberedelse af rytmisk-aktiv i Vitro Neonatal gnaver hjernestammen-rygmarven og tynd skive

Read Article