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En model af eksperimentel Steatosis In Vitro:Hepatocyt Cell Kultur i Lipid Overbelastning-Conditioned Medium
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JoVE Journal Medicine
A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium

En model af eksperimentel Steatosis In Vitro:Hepatocyt Cell Kultur i Lipid Overbelastning-Conditioned Medium

DOI:
10.3791/62543-v

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08:35 min

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May 18, 2021

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1Laboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina-UNAM,Hospital General de México “Dr. Eduardo Liceaga”

Chapters

  • 00:05Introduction
  • 00:55Standard and Conditioned Medium Preparation
  • 03:11Steatogenic Culture
  • 03:48Viability and Mortality Assessment
  • 04:44Lipid Staining with Oil-Red O
  • 05:46Morphometric Assessment of Lipid Contents
  • 06:29Results: Hepatocyte Culture in Steatogenic Conditions
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol er beregnet til at være et værktøj til at studere steatose og molekylær, biokemiske, cellulære ændringer produceret af overeksponering af hepatocytter til lipider in vitro.

Tags

Liver SteatosisIn VitroHepatocyte Cell CultureLipid OverloadPalmitateOleateSteatogenic MediumMild SteatosisSevere SteatosisRPMI 1640Fatty AcidsLipid-free BSAReproducibilityDiagnostic MarkersTherapeutic Targets

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