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Journal
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Immunology and Infection
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磷酸钙转染法制备假型H5禽流感病毒及抗体中和活性测定
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
Please note that all translations are automatically generated.
Click here for the English version.
磷酸钙转染法制备假型H5禽流感病毒及抗体中和活性测定
DOI:
10.3791/62626-v
•
07:15 min
•
November 22, 2021
•
Xiaoyan Chang*
1
,
Keru Zhou*
1
,
Yanguang Liu
2
,
Lian Duan
,
Lichun Zhang
,
Guoliang Zhang
,
Haikun Wang
,
Guiqin Wang
1
Nanjing Advanced Academy of Life and Health
,
2
Institute of Animal Bio-technology
,
Jilin Academy of Agricultural Sciences
,
3
National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital
,
Southern University of Science and Technology
,
4
CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences
,
University of Chinese Academy of Sciences
Chapters
00:04
Introduction
00:40
Pseudovirus Packaging with Calcium‐Phosphate Transfection
01:33
Detection of the HA, NA, and HIV‐1 P24 Protein Expression of Influenza Pseudovirus
02:24
Pseudovirus Titration
03:22
Luciferase Assay
04:06
Pseudovirus Neutralization (PN) Assay
05:16
Results: HPAI H5 Pseudovirus Preparations and Pseudovirus Neutralization Assays
06:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了假病毒包装和抗体中和活性测量的协议。
Tags
Pseudo-typed H5 Avian Influenza Viruses
Calcium Phosphate Transfection
Antibody Neutralizing Activity
Hemagglutinin Protein Expression
Pseudo Virus Titration
MDCK Cells
Luciferase Assay
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