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5-乙炔基-2'-脱氧尿苷/磷酸-组蛋白H3双标记方案用于 果蝇 神经干细胞的细胞周期进展分析
JoVE Journal
Biology
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JoVE Journal Biology
5-Ethynyl-2′-Deoxyuridine/Phospho-Histone H3 Dual-Labeling Protocol for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells
DOI:

09:34 min

May 04, 2021

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Chapters

  • 00:04Introduction
  • 00:40Dissection of Third Instar Larval Brains and 5-ethynyl-2 -deoxyuridine (EdU) Incorporation
  • 02:23EdU Detection, DNA Staining, and Mounting
  • 04:29Imaging
  • 05:52Image Analysis
  • 08:06Results: EdU and pH3 Dual-labeling for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells
  • 09:01Conclusion

Summary

Automatic Translation

使用5-乙炔基-2'-脱氧尿苷(EdU)和磷酸化组蛋白H3(pH3)标记进行细胞周期分析是一个多步骤程序,可能需要广泛的优化。在这里,我们提出了一个详细的方案,描述了该过程的所有步骤,包括图像分析和定量,以区分不同细胞周期阶段的细胞。

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