A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Introduction
- 00:56Culturing and Maintenance of THP‐1 Monocyte-like Cells
- 02:59Seeding of THP‐1 cells and Differentiation into M0 Macrophages
- 04:01Polarization of M0 Macrophages into M2‐like Macrophages
- 04:39Detaching and Harvesting Macrophages for Flow Cytometry
- 05:14Results: Macrophage Differentiation and Polarization into an M2‐Like Phenotype
- 05:56Conclusion
