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Immunology and Infection
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Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

DOI:
10.3791/62677-v

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06:14 min

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May 13, 2021

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, , , ,
1Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy,Texas Tech University Health Sciences Center, 2Molecular Neurosurgery Laboratory and the Brain Tumor Research Center, Department of Neurosurgery,Massachusetts General Hospital and Harvard Medical School

Chapters

  • 00:04Introduction
  • 01:06Oncolytic Herpes Simplex Virus (oHSV) Growth
  • 02:46Oncolytic Herpes Simplex Virus (oHSV) Purification
  • 05:10Results: Microscopic Analysis of Vero Cells Infected with oHSV
  • 05:44Conclusion

Summary

Automatic Translation

Automatic Translation

In this manuscript, we describe a simple method of growth, purification, and titration of the oncolytic herpes simplex virus for preclinical use.

Tags

Keywords: Oncolytic Herpes Simplex VirusCancer ImmunotherapyVirus PropagationVirus PurificationVirus TitrationBiosafety Level TwoVirus StockAnticancer Immune ResponseVirus-based Cancer ImmunotherapyWild-type Herpes Simplex VirusGenetically Engineered Herpes Simplex VirusT-150 Square Centimeter FlasksDPBSIFCSDMEMCell ScraperCentrifugationVirus BufferBenzonase NucleaseVirus Titer

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