/

Immunology and Infection

/

单纯疱疹病毒的生长、净化和滴定

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Please note that all translations are automatically generated. Click here for the English version.

单纯疱疹病毒的生长、净化和滴定

DOI:

10.3791/62677-v

•

06:14 min

•

May 13, 2021

•

Hong-My Nguyen*¹, Naresh Sah*¹, Melissa R. M. Humphrey, Samuel D. Rabkin, Dipongkor Saha

¹Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy,Texas Tech University Health Sciences Center, ²Molecular Neurosurgery Laboratory and the Brain Tumor Research Center, Department of Neurosurgery,Massachusetts General Hospital and Harvard Medical School

Chapters

00:04Introduction
01:06Oncolytic Herpes Simplex Virus (oHSV) Growth
02:46Oncolytic Herpes Simplex Virus (oHSV) Purification
05:10Results: Microscopic Analysis of Vero Cells Infected with oHSV
05:44Conclusion

Summary

Automatic Translation

在本手稿中，我们描述了一个简单的方法的生长，纯化和滴定肿瘤疱疹简单病毒的药物前使用。

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Epstein - Barr病毒生长转化的淋巴细胞系的建立

神经元的初步培养体系的研究疱疹病毒潜伏期和活化

流感病毒从感染细胞的染色质的核糖核蛋白复合物的亲和纯化

鼠表皮离体感染单纯疱疹病毒1型

中性粒细胞分离和分析，以确定他们的淋巴瘤细胞的敏感性对治疗药物的作用

重组原型泡沫病毒整合纯化过程中核酸污染的检测与去除

泡沫病毒 Intasomes 原型的组装与纯化

免疫荧光共聚焦显微镜对单纯疱疹病毒1蛋白核细胞质转移的时间分析

猪全血表达编码和非编码 rna 类的鉴定

重组呼吸道合胞病毒的生成、扩增和滴定

Read Article