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Transduction transitoire des formes strobilées d’Echinococcus granulosus
JoVE Journal
Genetics
Author Produced
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JoVE Journal Genetics
Transient Transduction of the Strobilated Forms of Echinococcus granulosus
DOI:

13:25 min

September 16, 2022

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Chapters

  • 00:15Introduction
  • 01:34Collecting Hydatid Cysts
  • 03:06Biphasic Cultivation of Protoscoleces of E. granulosus to Obtain Adult Worms
  • 03:59Monophasic Cultivation of Protoscoleces of E. granulosus
  • 04:27Cell Culture for Virus Production and Preparation
  • 05:06Production and Preparation of the Virus with the Third-Generation Lentiviral Vectors
  • 07:49Transient Transduction of Different Stages of E. granulosus with the Virus
  • 09:44Representative Results
  • 11:28Conclusion

Summary

Automatic Translation

Nous décrivons une technique de transduction transitoire rapide à différents stades de développement d’Echinococcus granulosus en utilisant des vecteurs lentiviraux de troisième génération.

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