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Journal
/
Genetics
/
エキノコッカス顆粒症
のストロビレー化形態の一過性形質導入
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Genetics
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JoVE Journal
Genetics
Transient Transduction of the Strobilated Forms of
Echinococcus granulosus
Please note that all translations are automatically generated.
Click here for the English version.
エキノコッカス顆粒症
のストロビレー化形態の一過性形質導入
DOI:
10.3791/62783-v
•
13:25 min
•
September 16, 2022
•
Mohammad Ali Mohammadi
,
Ali Afgar
,
Ashkan Faridi
,
Seyed Mohammad Mousavi
,
Ali Derakhshani
,
Mehdi Borhani
,
Majid Fasihi Harandi
1
Student Research Committee, School of Medicine
,
Kerman University of Medical Sciences
,
2
Research Center for Hydatid Disease in Iran
,
Kerman University of Medical Sciences
,
3
Department of Parasitology and Mycology, Faculty of Medicine
,
Kurdistan University of Medical Sciences
,
4
State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine
,
Jilin University
Chapters
00:15
Introduction
01:34
Collecting Hydatid Cysts
03:06
Biphasic Cultivation of Protoscoleces of
E. granulosus
to Obtain Adult Worms
03:59
Monophasic Cultivation of Protoscoleces of
E. granulosus
04:27
Cell Culture for Virus Production and Preparation
05:06
Production and Preparation of the Virus with the Third-Generation Lentiviral Vectors
07:49
Transient Transduction of Different Stages of
E. granulosus
with the Virus
09:44
Representative Results
11:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、第3世代レンチウイルスベクターを用いた
エキノコッカス顆粒症
のさまざまな発生段階における迅速な一過性形質導入技術について記載する。
Tags
Echinococcus Granulosus
Cystic Echinococcosis
Hydatid Disease
Zoonotic Parasitic Disease
Protoscoleces
Strobilated Worms
Transient Transduction
Lentiviral Vectors
Gene Transfer
Cestode Parasites
Gene Evaluation System
Viral Delivery System
GFP Reporter Gene
Biphasic Cultivation
Article
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