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In vivo Immunogenicitetsscreening af tumorafledte ekstracellulære vesikler ved flowcytometri af milt-T-celler
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JoVE Journal Cancer Research
In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

In vivo Immunogenicitetsscreening af tumorafledte ekstracellulære vesikler ved flowcytometri af milt-T-celler

DOI:
10.3791/62811-v

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08:02 min

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September 23, 2021

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, ,
1Department of Medicine III, School of Medicine,Technical University of Munich, 2Center for Translational Cancer Research (TranslaTUM), School of Medicine,Technical University of Munich, 3Department of Internal Medicine III,University Hospital Regensburg, 4National Centre for Tumor Diseases WERA

Chapters

  • 00:05Introduction
  • 00:48Generation and Isolation of Extracellular Vesicles (EVs) Derived from Tumor Cells After Chemotherapy Exposure
  • 02:59Immunization of C57BL/6 Mice with Extracellular Vesicles (EVs) and Flow Cytometry Analysis of Splenic T Cells
  • 06:01Results: The Assessment of the Immunogenicity of Tumor-Derived Extracellular Vesicles (EVs)
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskript beskriver, hvordan man vurderer in vivo-immunogenicitet af tumorcelleafledte ekstracellulære vesikler (IV'er) ved hjælp af flowcytometri. ELBILER afledt af tumorer, der gennemgår behandlingsinduceret immunogen celledød, synes særligt relevante i tumorimmunovervågning. Denne protokol eksemplificerer vurderingen af oxaliplatin-inducerede immunstimulerende tumor-ELBILER, men kan tilpasses forskellige indstillinger.

Tags

Keywords: – Tumor-derived Extracellular Vesicles – Flow Cytometry – Splenic T Cells – In Vivo Immunogenicity Screening – B16 Melanoma Cells – Ovalbumin – Oxaliplatin – Ultracentrifugation – EV Isolation – Exosome Isolation Reagent – Immunization

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