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Medicine
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用于免疫组化的合成抗原对照
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Medicine
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JoVE Journal
Medicine
Synthetic Antigen Controls for Immunohistochemistry
Please note that all translations are automatically generated.
Click here for the English version.
用于免疫组化的合成抗原对照
DOI:
10.3791/62819-v
•
09:30 min
•
August 23, 2021
•
Charles A. Havnar
,
Kathy J. Hötzel
,
Charles A. Jones
,
Carmina M. Espiritu
,
Linda K. Rangell
,
Franklin V. Peale
1
Research Pathology
,
Genentech, Inc.
Chapters
00:05
Introduction
00:46
Preparation of Stock Solutions and Tools
02:26
Preparation and Dilutions of Peptides
04:18
Preparing BSA-Peptide Gels
05:37
Trimming, Processing, Embedding BSA Gels
07:00
Pilot Evaluation of the Peptide Dilution Series and BSA Gel Tissue Microarray Construction
08:07
Results: Staining of ERBB2/HER2 Peptide TMA with ERBB2/HER2 1+ and 3+ Cell Lines
08:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作记录了一种为免疫组化创建合成抗原对照的简单方法。该技术适用于各种浓度的抗原。这些样品为评估测定内和测定间的性能和再现性提供了参考。
Tags
Keywords: BSA Antigen Gel
IHC Standards
Reproducible Controls
Calibration
Standardization
Peptide
Protein
Formaldehyde
PBS
Heat Block
Gelation
Peptide Stock Solution
Molecular Weight
Purity
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