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用于免疫组化的合成抗原对照
JoVE Journal
Medicine
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JoVE Journal Medicine
Synthetic Antigen Controls for Immunohistochemistry
DOI:

09:30 min

August 23, 2021

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Chapters

  • 00:05Introduction
  • 00:46Preparation of Stock Solutions and Tools
  • 02:26Preparation and Dilutions of Peptides
  • 04:18Preparing BSA-Peptide Gels
  • 05:37Trimming, Processing, Embedding BSA Gels
  • 07:00Pilot Evaluation of the Peptide Dilution Series and BSA Gel Tissue Microarray Construction
  • 08:07Results: Staining of ERBB2/HER2 Peptide TMA with ERBB2/HER2 1+ and 3+ Cell Lines
  • 08:46Conclusion

Summary

Automatic Translation

这项工作记录了一种为免疫组化创建合成抗原对照的简单方法。该技术适用于各种浓度的抗原。这些样品为评估测定内和测定间的性能和再现性提供了参考。

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