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Immunology and Infection
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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
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Immunology and Infection
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JoVE Journal Immunology and Infection
User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

DOI:
10.3791/62832-v

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07:56 min

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July 29, 2021

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, , ,
1Molecular Biophysics Unit,Indian Institute of Science, 2Gatan Inc.

Chapters

  • 00:04Introduction
  • 00:40Data Acquisition with Latitude-S
  • 03:34Fine Alignment
  • 04:20Data Acquisition Procedure
  • 05:36Results: Automated Data Acquisition Using Latitude-S for Single-Particle Cryo-Electron Microscopy
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

Single-particle cryo-electron microscopy demands a suitable software package and user-friendly pipeline for high-throughput automatic data acquisition. Here, we present the application of a fully automated image acquisition software package, Latitude-S, and a practical pipeline for data collection of vitrified biomolecules under low-dose conditions.

Tags

Keywords: 1. Automated Data Acquisition Software 2. Single-particle Cryo-electron Microscopy 3. Structural Characterization 4. Biological Macromolecules 5. Pathogen Biology 6. Latitude-S 7. Technique Manager 8. Single-Particle Automated Data Collection 9. TEM Condition 10. Camera Condition 11. Atlas State 12. Grid State 13. Hole State 14. Focus State 15. Data State 16. Defocus Values 17. Microscope Settings 18. Imaging Optics 19. Illumination Conditions 20. Camera Exposure Time

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