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萌芽酵母酿酒酵母中软骨蛋白定位评估
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
DOI:

08:55 min

July 19, 2021

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Chapters

  • 00:04Introduction
  • 00:33Yeast Cells Growth
  • 01:37Isolation of Highly Purified Mitochondria
  • 02:03Sub-Mitochondrial Fractionation: Hypotonic Swelling Using Proteinase K
  • 04:01Sub-Mitochondrial Fractionation: Sonication and Carbonate Extraction
  • 06:31Results: Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
  • 08:14Conclusion

Summary

Automatic Translation

尽管最近取得了进展,但许多酵母线粒体蛋白仍然具有完全未知的功能。该协议提供了一种简单可靠的方法来确定蛋白质的膜软骨定位,这对于阐明其分子功能至关重要。

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