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Bioengineering
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携带人多能干细胞球体的核壳微胶囊的微流体制造
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Bioengineering
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JoVE Journal
Bioengineering
Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
Please note that all translations are automatically generated.
Click here for the English version.
携带人多能干细胞球体的核壳微胶囊的微流体制造
DOI:
10.3791/62944-v
•
10:51 min
•
October 13, 2021
•
Kihak Gwon*
1
,
Hye Jin Hong*
1
,
Alan M. Gonzalez-Suarez
,
Gulnaz Stybayeva
,
Alexander Revzin
1
Department of Physiology and Biomedical Engineering
,
Mayo Clinic
Chapters
00:04
Introduction
01:31
Solution Preparation
04:21
Experimental Setup
07:05
hPSC Culture and Analysis in Microcapsules
08:31
Results: Analysis of Characterization of Microcapsules and Encapsulation of hPSCs
09:47
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了使用同轴流聚焦装置封装人多能干细胞(hPSC)。我们证明,这种微流体封装技术能够有效地形成hPSC球体。
Tags
Keywords: Microfluidic Fabrication
Core-shell Microcapsules
Human Pluripotent Stem Cell Spheroids
3D Cell Culture
Stem Cell Encapsulation
Scalable Stem Cell Cultivation
Shear Protection
Cost-effective Cellular Therapy
PEG
Density Gradient Media
Mineral Oil
Surfactant
DTT
TEA
PF127
Cross-linker Emulsion
Shielding Oil
PEG4 Maleimide
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