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Bioengineering
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ヒト多能性幹細胞スフェロイドを担持したコアシェル型マイクロカプセルのマイクロ流体作製
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Bioengineering
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JoVE Journal
Bioengineering
Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
Please note that all translations are automatically generated.
Click here for the English version.
ヒト多能性幹細胞スフェロイドを担持したコアシェル型マイクロカプセルのマイクロ流体作製
DOI:
10.3791/62944-v
•
10:51 min
•
October 13, 2021
•
Kihak Gwon*
1
,
Hye Jin Hong*
1
,
Alan M. Gonzalez-Suarez
,
Gulnaz Stybayeva
,
Alexander Revzin
1
Department of Physiology and Biomedical Engineering
,
Mayo Clinic
Chapters
00:04
Introduction
01:31
Solution Preparation
04:21
Experimental Setup
07:05
hPSC Culture and Analysis in Microcapsules
08:31
Results: Analysis of Characterization of Microcapsules and Encapsulation of hPSCs
09:47
Conclusion
Summary
Automatic Translation
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Automatic Translation
この記事では、同軸流集束装置を用いたヒト多能性幹細胞(hPSC)のカプセル化について説明する。我々は、このマイクロ流体封止技術がhPSCスフェロイドの効率的な形成を可能にすることを実証する。
Tags
Keywords: Microfluidic Fabrication
Core-shell Microcapsules
Human Pluripotent Stem Cell Spheroids
3D Cell Culture
Stem Cell Encapsulation
Scalable Stem Cell Cultivation
Shear Protection
Cost-effective Cellular Therapy
PEG
Density Gradient Media
Mineral Oil
Surfactant
DTT
TEA
PF127
Cross-linker Emulsion
Shielding Oil
PEG4 Maleimide
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