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开发移动式线粒体生理学实验室，用于在野外测量线粒体能量学

JoVE Journal
Biology

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JoVE Journal Biology

Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field

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开发移动式线粒体生理学实验室，用于在野外测量线粒体能量学

DOI:

10.3791/62956-v

•

08:54 min

•

August 27, 2021

•

Hailey A. Parry, Kang Nian Yap, Geoffrey E. Hill, Wendy R. Hood, L. Bruce Gladden, Michael Eddy, Andreas N. Kavazis

¹School of Kinesiology,Auburn University, ²Department of Biological Sciences,Auburn University, ³Department of Electrical and Computer Engineering,Auburn University

Chapters

00:04Introduction
00:56Isolation of Mitochondria
03:53Mitochondrial Respiration Measurements
06:47Results: Mitochondrial Respiration Rates from Wild-Derived House Mice (Mus musculus) Hindlimb Skeletal Muscle
08:07Conclusion

Summary

Automatic Translation

我们设计并建造了一个移动实验室，以测量在野外捕获的野生动物分离的线粒体的呼吸速率。在这里，我们描述了移动线粒体实验室的设计和装备以及相关的实验室协议。

Tags

Keywords: Mobile Mitochondrial Laboratory Mitochondrial Energetics Mitochondrial Respiration Field Physiology Ecological And Evolutionary Context Mitochondrial Isolation Mitochondrial Respiration Chambers Mobile Lab Setup

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