Journal
/
Immunology and Infection
/
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

DOI:
10.3791/62984-v

•

06:55 min

•

October 19, 2021

•

, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

Chapters

  • 00:05Introduction
  • 00:39Activating Human Primary T Lymphocytes
  • 01:55Extracellular Flux Assay
  • 04:52Results: Optimizing Mitochondrial Modulator Concentrations and Cell Numbers for Monitoring Mitochondrial Respiration in Human Primary T Cells
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

Metabolic adaptation is fundamental for T cells as it dictates differentiation, persistence, and cytotoxicity. Here, an optimized protocol for monitoring mitochondrial respiration in ex vivo cytokine-differentiated human primary T cells is presented.

Tags

Mitochondrial RespirationT CellCytokineSeahorse MachineExtracellular Flux AssayCell-TakXF Cell Culture PlateAssay MediaGlucosePyruvateGlutamine

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62984?article_type=v

Retroviral Transduction of T-cell Receptors in Mouse T-cells
check_url/62984?article_type=v

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
check_url/62984?article_type=v

piggyBac Transposon System Modification of Primary Human T Cells
check_url/62984?article_type=v

Real-time Live Imaging of T-cell Signaling Complex Formation
check_url/62984?article_type=v

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
check_url/62984?article_type=v

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
check_url/62984?article_type=v

Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue
check_url/62984?article_type=v

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
check_url/62984?article_type=v

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
check_url/62984?article_type=v

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

Read Article