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Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes
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Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes

Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes

DOI:
10.3791/62989-v

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06:12 min

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September 30, 2021

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, , , ,
1Department of Cell Biology,The Johns Hopkins University School of Medicine, 2Tufts School of Medicine, 3Department of Biomedical Sciences,Idaho College of Osteopathic Medicine

Chapters

  • 00:04Introduction
  • 00:46Salivary Gland Dissection
  • 02:01Fixation and Immunostaining
  • 03:25Mounting Stained Glands for Microscopy
  • 04:25Results: Morphology of L4 Stage Mosquito and Salivary Gland Dissected from Mosquito Head
  • 05:41Conclusion

Summary

Automatic Translation

Automatic Translation

The adult mosquito salivary gland (SG) is required for the transmission of all mosquito-borne pathogens to their human hosts, including viruses and parasites. This video demonstrates efficient isolation of the SGs from the larval (L4) stage Anopheles gambiae mosquitoes and preparation of the L4 SGs for further analysis.

Tags

Keywords: Salivary GlandsAnopheles GambiaeMosquito LarvaeDissectionImmunostainingDisease TransmissionMalarial ParasitesRab 11Alexa Fluor 488Nile RedHoechst

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