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Biology
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冈
比亚按蚊
幼虫唾液腺的解剖和免疫染色
JoVE Journal
Biology
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JoVE Journal
Biology
Dissection and Immunostaining of Larval Salivary Glands from
Anopheles gambiae
Mosquitoes
Please note that all translations are automatically generated.
Click here for the English version.
冈
比亚按蚊
幼虫唾液腺的解剖和免疫染色
DOI:
10.3791/62989-v
•
06:12 min
•
September 30, 2021
•
Michelle Z. Chiu
2
,
Steven Lannon
,
Marisol Luchetti
,
Michael B. Wells
,
Deborah J. Andrew
1
Department of Cell Biology
,
The Johns Hopkins University School of Medicine
,
2
Tufts School of Medicine
,
3
Department of Biomedical Sciences
,
Idaho College of Osteopathic Medicine
Chapters
00:04
Introduction
00:46
Salivary Gland Dissection
02:01
Fixation and Immunostaining
03:25
Mounting Stained Glands for Microscopy
04:25
Results: Morphology of L4 Stage Mosquito and Salivary Gland Dissected from Mosquito Head
05:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
成年蚊子唾液腺(SG)是将所有蚊子传播的病原体(包括病毒和寄生虫)传播给人类宿主所必需的。该视频演示了从幼虫(L4)阶段冈
比亚按蚊
中有效分离SG以及L4 SGs的制备以进行进一步分析。
Tags
Keywords: Salivary Glands
Anopheles Gambiae
Mosquito Larvae
Dissection
Immunostaining
Disease Transmission
Malarial Parasites
Rab 11
Alexa Fluor 488
Nile Red
Hoechst
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