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Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
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Chapters
- 00:05Introduction
- 00:37Preparation of Microinjection Slides
- 01:19Collection of Young Adult Strongyloides for Microinjection
- 03:19Preparing the Microscope and Breaking the Needle
- 05:27Microinjecting Strongyloides
- 07:22Collecting and Screening F1 Larvae to Recover Transgenics/Knockouts
- 08:31Results: Generating Transgenics and Knockouts in Strongyloides Species Using Intragonadal Microinjection
- 09:04Conclusion
The functional genomic toolkit for the parasitic nematodes Strongyloides stercoralis and Strongyloides ratti includes transgenesis, CRISPR/Cas9-mediated mutagenesis, and RNAi. This protocol will demonstrate how to use intragonadal microinjection to introduce transgenes and CRISPR components into S. stercoralis and S. ratti.
