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Generering af transgenik og knockouts i Strongyloides Species ved mikroinjektion
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Chapters
- 00:05Introduction
- 00:37Preparation of Microinjection Slides
- 01:19Collection of Young Adult Strongyloides for Microinjection
- 03:19Preparing the Microscope and Breaking the Needle
- 05:27Microinjecting Strongyloides
- 07:22Collecting and Screening F1 Larvae to Recover Transgenics/Knockouts
- 08:31Results: Generating Transgenics and Knockouts in Strongyloides Species Using Intragonadal Microinjection
- 09:04Conclusion
Det funktionelle genomiske værktøjssæt til de parasitære nematoder Strongyloides stercoralis og Strongyloides ratti omfatter transgenese, CRISPR/Cas9-medieret mutagenese og RNAi. Denne protokol vil demonstrere, hvordan man bruger intragonadal mikroinjektion til at introducere transgener og CRISPR-komponenter i S. stercoralis og S. ratti.
