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El ensayo basado en microscopía para estudiar y analizar los endosomas de reciclaje utilizando el tráfico de SNARE
JoVE Journal
Biology
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JoVE Journal Biology
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
DOI:

08:51 min

February 12, 2022

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Chapters

  • 00:06Introduction
  • 01:04Seeding of Mouse Melanocytes on Pre-Treated Coverslips
  • 01:42Transfection of Cells with the STX13 Plasmids
  • 02:39Fixation of the Cells
  • 03:11Immunostaining of the Cells
  • 05:05Results: Quantification of Overlap Between the RE Localized Proteins and Melanosomes
  • 07:25Results: Quantification of Recycling Endosomes’ Tublar Number and Length
  • 07:56Conclusion

Summary

Automatic Translation

Los endosomas de reciclaje forman parte de la red tubular endosomal. Aquí presentamos un método para cuantificar la dinámica del reciclaje de endosomas utilizando GFP-STX13 como marcador de orgánulos.

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