Journal
/
Medicine
/
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

DOI:
10.3791/63107-v

•

08:23 min

•

February 16, 2022

•

, , ,
1Department of Anesthesia, Critical Care and Pain Medicine,Massachusetts General Hospital, 2Department of Pediatrics,Massachusetts General Hospital, 3Harvard Medical School

Chapters

  • 00:04Introduction
  • 01:07Setup Preparation for Nitric Oxide Consumption by Cell-Free Hemoglobin
  • 01:33Starting the Chemiluminescence Detector and Preparing the Purge Vessel
  • 03:56Performing the Experiment
  • 05:57Results: Nitric Oxide-Consumption by Cell-Free Hemoglobin
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present protocols for detecting nitric oxide and its biologically relevant derivatives using chemiluminescence-based assays with high sensitivity.

Tags

Keywords: ChemiluminescenceNitric OxideDETA-NONOateOxygenPurge VesselInert GasMetabolitesAssayDetectionTherapeutic GasAntimicrobialAirway Infectious DiseasesLiquid Phase Assays

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63107?article_type=v

Production and Detection of Reactive Oxygen Species (ROS) in Cancers
check_url/63107?article_type=v

Analytical Techniques for Assaying Nitric Oxide Bioactivity
check_url/63107?article_type=v

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
check_url/63107?article_type=v

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring
check_url/63107?article_type=v

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
check_url/63107?article_type=v

Polyacrylamide Gels for Invadopodia and Traction Force Assays on Cancer Cells
check_url/63107?article_type=v

Boldness, Aggression, and Shoaling Assays for Zebrafish Behavioral Syndromes
check_url/63107?article_type=v

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
check_url/63107?article_type=v

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
check_url/63107?article_type=v

A Novel Inhalation Mask System to Deliver High Concentrations of Nitric Oxide Gas in Spontaneously Breathing Subjects

Read Article