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Journal / Biochemistry / Cellulære membranaffinitetskromatografikolonner…
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JoVE Journal Biochemistry
Cellulære membranaffinitetskromatografikolonner til identifikation af specialiserede plantemetabolitter, der interagerer med immobiliseret tropomyosinkinasereceptor B
 
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Cellulære membranaffinitetskromatografikolonner til identifikation af specialiserede plantemetabolitter, der interagerer med immobiliseret tropomyosinkinasereceptor B

Article DOI: 10.3791/63118-v • 11:44 min • January 19th, 2022
January 19th, 2022 •
1,2, 1, 1, 1, 1
1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

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Protokollen beskriver fremstillingen af cellemembranaffinitetskromatografi (CMAC) kolonner med immobiliserede cellemembranfragmenter indeholdende funktionelle transmembrantropomyosinkinasereceptor B-proteiner. Anvendelsen af CMAC-kolonner til identifikation af specialiserede plantemetabolitter, der interagerer med disse receptorer og er til stede i komplekse naturlige blandinger, forklares også.

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