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Cancer Research

Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection

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Cancer Research

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JoVE Journal Cancer Research

Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection

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Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection

DOI:

10.3791/63123-v

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05:49 min

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November 01, 2021

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Tiziano Lottini, Matteo Buonamici, Claudia Duranti, Annarosa Arcangeli

¹Department of Experimental and Clinical Medicine, Section of Internal Medicine,University of Florence

Chapters

00:05Introduction
01:00Mice Preparation for Ultrasound-Guided Injection (US-GI)
02:23Injection of PANC1 Cells in the Pancreas by Ultrasound-Guided Injection (US-GI) Method
04:17Results: Development of Orthotopic Pancreatic Cancer (PCa) Xenograft in the Mouse Pancreas
05:06Conclusion

Summary

Automatic Translation

We present a protocol to generate a minimally invasive orthotopic pancreatic cancer model by ultrasound-guided injection of human pancreatic cancer cells and the subsequent monitoring of tumor growth in vivo by ultrasound imaging.

Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection

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