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离体小鼠通过门静脉进行肝灌注

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse

Please note that all translations are automatically generated. Click here for the English version.

离体小鼠通过门静脉进行肝灌注

DOI:

10.3791/63154-v

•

05:30 min

•

March 09, 2022

•

Anthony G. Giacalone, Matthew E. Merritt, Mukundan Ragavan

¹Department of Biochemistry and Molecular Biology,University of Florida

Chapters

00:04Introduction
01:01Celiotomy
01:44Cannulation of Portal Vein
02:50Resection of Liver Post Portal Vein Cannulation
04:18Results: Liver Function Assessment by Oxygen Consumption and Flow Rate Measurement
04:45Conclusion

Summary

Automatic Translation

该协议描述了一种通过门静脉灌注切除完整小鼠肝脏以进行代谢研究的简单方法。

Tags

Keywords: Ex Vivo Hepatic Perfusion Portal Vein Mouse Liver Metabolism Disease Models NAFLD NASH Pharmaceutical Assessment Anesthesia Celiotomy Portal Vein Cannulation Catheter Suture Syringe

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