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Developmental Biology
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小鼠输卵管的显微解剖和解离:个体片段鉴定和单细胞分离
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation
Please note that all translations are automatically generated.
Click here for the English version.
小鼠输卵管的显微解剖和解离:个体片段鉴定和单细胞分离
DOI:
10.3791/63168-v
•
07:44 min
•
November 04, 2021
•
Kelly C. Radecki
,
Mary Y. Lorenson
,
David G. Carter
,
Ameae M. Walker
1
Division of Biomedical Sciences, School of Medicine
,
University of California, Riverside
,
2
Microscopy and Imaging Core Facility
,
University of California, Riverside
Chapters
00:05
Introduction
01:40
Macrodissection of The Ovary, Oviduct, and Uterus
03:28
Microdissection and Segmentation of the Oviduct
04:15
Oviduct Dissociation
04:45
Results: Oviduct Cell Dissociation and Quality Assessment of Segmental RNA
06:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一种小鼠输卵管显微解剖方法,该方法允许在保持RNA完整性的同时收集单个片段。此外,描述了非酶促卵管细胞解离过程。这些方法适用于功能不同的输卵管节段和解离的输卵管细胞的后续基因和蛋白质分析。
Tags
Microdissection
Dissociation
Murine Oviduct
Segment Identification
Single Cell Isolation
Ampulla
Infundibulum
Stromal Cells
Epithelial Cells
Immune Cells
Smooth Muscle Cells
In Vitro Fertilization
Malignant Transformation
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