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鸡重组肢体测定,以了解细胞分化的形态发生,图案化和早期步骤
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
DOI:

08:08 min

January 12, 2022

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Chapters

  • 00:04Introduction
  • 00:38Embryo Incubation and Determination of Viability
  • 01:06Obtaining Limb Mesodermal Cells to Fill Ectoderms
  • 04:11Obtaining the Limb Ectoderms
  • 05:21Assembling Mesodermal Cells Inside the Ectodermal Hull
  • 06:04Transplantation of the Filled Ectoderm into a Host Embryo
  • 06:51Results: Morphology and Patterning of Recombinant Limbs
  • 07:40Conclusion

Summary

Automatic Translation

重组肢体是一种强大的实验模型,可以研究细胞分化过程和胚胎信号影响下的模式生成。该协议提出了一种详细的方法,用于生成具有鸡肢 - 中胚层细胞的重组肢体,可适应从不同生物体获得的其他细胞类型。

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