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Journal
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Bioengineering
/
纸基脚趾开关诊断开发和患者验证的设计到实施研究
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics
Please note that all translations are automatically generated.
Click here for the English version.
纸基脚趾开关诊断开发和患者验证的设计到实施研究
DOI:
10.3791/63223-v
•
10:42 min
•
June 17, 2022
•
Katariina Jaenes*
1
,
Severino Jefferson Ribeiro da Silva*
1,2
,
Justin R. J. Vigar*
1
,
Kaiyue Wu
4
,
Masoud Norouzi
,
Pouriya Bayat
,
Margot Karlikow
,
Seray Cicek
,
Yuxiu Guo
,
Alexander A. Green
4
,
Lindomar Pena
,
Keith Pardee
5
1
Leslie Dan Faculty of Pharmacy
,
University of Toronto
,
2
Laboratory of Virology and Experimental Therapy (LAVITE), Department of Virology, Aggeu Magalhães Institute (IAM)
,
Oswaldo Cruz Foundation (Fiocruz)
,
3
Department of Biomedical Engineering
,
Boston University
,
4
Molecular Biology, Cell Biology & Biochemistry Program, Graduate School of Arts and Sciences
,
Boston University
,
5
Department of Mechanical and Industrial Engineering
,
University of Toronto
Chapters
00:04
Introduction
01:07
Computational Design of Toehold Switches
02:45
Construction of Toehold Switches by PCR
03:32
Initial Screening of the Switches
05:16
NASBA Primer Screening and Sensitivity
06:55
RT-qPCR for Zika Virus Detection
08:08
Results: Evaluation of Toehold Switches for Paper-Based Zika Sensors
10:01
Conclusion
Summary
Automatic Translation
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Automatic Translation
获得分散、低成本和高容量的诊断方法,这些诊断方法可以部署到社区中进行分散检测,这对于应对全球卫生危机至关重要。本手稿描述了如何为可以使用便携式光学阅读器检测的病毒RNA序列构建基于纸张的诊断方法。
Tags
Paper-based Diagnostics
Toehold Switch
Gene Circuit
Nucleic Acid Detection
Cell-free Diagnostics
Zika Virus
MATLAB Design Software
NCBI BLAST
PCR
Agarose Gel Electrophoresis
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