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Kultivierung von Lymphozyten in simulierter Schwerelosigkeit mit einem rotierenden Zellkultursystem
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1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine,University of Toronto, 2Krembil Research Institute, Toronto Western Hospital,University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital,Sinai Health Systems, 4Medical Oncology and Hematology,Princess Margaret Cancer Centre
Chapters
- 00:04Introduction
- 00:48Preparation of Vessels for Cell Culture
- 01:43Loading Vessels with Stock Cell Culture
- 04:42Removing Bubbles from the Vessels
- 05:47Attaching the Vessel to the Rotating Base
- 06:36Treatment and Harvesting Cells from the Vessels
- 08:06Results: Application of the Rotary Cell Culture System to Study the Effects of Simulated Microgravity on Lymphocytes
- 08:55Conclusion
Dies ist eine Schritt-für-Schritt-Anleitung für die Verwendung eines kommerziell erhältlichen rotierenden Zellkultursystems zur Kultivierung von Lymphozyten in simulierter Schwerelosigkeit mit speziellen Einweg-Kulturgefäßen. Diese Kultivierungsmethode kann auf jede Suspensionszellkultur angewendet werden.
Tags
LymphocytesSimulated MicrogravityRotary Cell Culture SystemImmune CellsSpace EnvironmentHuman PhysiologyMolecular LevelEconomically FeasibleCell CultureControlTreatmentComplete MediaIncubatorFlask ControlT25 Suspension Culture FlaskStock Cell CultureSyringe Port CapsStopcocksFill PortSerological PipetteVacuum System
