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Bir Döner Hücre Kültür Sistemi Kullanarak Simüle Edilmiş Mikrogravitede Lenfositlerin Kültürlenmesi
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1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine,University of Toronto, 2Krembil Research Institute, Toronto Western Hospital,University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital,Sinai Health Systems, 4Medical Oncology and Hematology,Princess Margaret Cancer Centre
Chapters
- 00:04Introduction
- 00:48Preparation of Vessels for Cell Culture
- 01:43Loading Vessels with Stock Cell Culture
- 04:42Removing Bubbles from the Vessels
- 05:47Attaching the Vessel to the Rotating Base
- 06:36Treatment and Harvesting Cells from the Vessels
- 08:06Results: Application of the Rotary Cell Culture System to Study the Effects of Simulated Microgravity on Lymphocytes
- 08:55Conclusion
Bu, özel tek kullanımlık kültür kapları kullanarak simüle edilmiş mikro yerçekiminde lenfositleri kültürlemek için ticari olarak temin edilebilen bir döner hücre kültürü sistemi kullanmak için adım adım bir kılavuzdur. Bu kültürleme yöntemi herhangi bir süspansiyon tipi hücre kültürüne uygulanabilir.
Tags
LymphocytesSimulated MicrogravityRotary Cell Culture SystemImmune CellsSpace EnvironmentHuman PhysiologyMolecular LevelEconomically FeasibleCell CultureControlTreatmentComplete MediaIncubatorFlask ControlT25 Suspension Culture FlaskStock Cell CultureSyringe Port CapsStopcocksFill PortSerological PipetteVacuum System
