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Generazione ed espansione delle linee tumorali primarie e maligne del mesotelioma pleurico
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
DOI:

08:01 min

April 21, 2022

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Chapters

  • 00:05Introduction
  • 00:45Digestion of Tumor Tissue
  • 03:16Generation of Primary Tumor Cell Line
  • 04:47Characterization and Banking of Established Primary Tumor Cell Line
  • 06:13Results: Determination of Fibroblast Contamination of Cell Culture and Flow Cytometry Surface Staining of Cell Lines
  • 07:12Conclusion

Summary

Automatic Translation

L'obiettivo di questo documento di metodo è dimostrare una metodologia robusta e riproducibile per l'arricchimento, la generazione e l'espansione di linee cellulari tumorali primarie da mesotelioma pleurico resecato chirurgicamente.

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