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Journal
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Developmental Biology
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アメリカのゴキブリにおけるRNA干渉の応用
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Developmental Biology
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JoVE Journal
Developmental Biology
Applications of RNA Interference in American Cockroach
Please note that all translations are automatically generated.
Click here for the English version.
アメリカのゴキブリにおけるRNA干渉の応用
DOI:
10.3791/63380-v
•
06:32 min
•
December 17, 2021
•
Liang Li*
1
,
Andi Jing*
1
,
Minxin Xie
,
Sheng Li
2
,
Chonghua Ren
2
1
Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences
,
South China Normal University
,
2
Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology
,
South China Normal University
Chapters
00:04
Introduction
01:19
Feeding of
P. americana
and Selection of the Nymphs in Proper Instar
01:42
Transcription and Synthesis of dsRNA In Vitro
03:39
Loading dsRNA Solution into the Syringe
04:08
Injecting dsRNA to
P. americana
05:00
Results: Efficiency and Application of RNA Interference
05:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
本プロトコルは、
P. americana
におけるRNAi操作技術のための段階的なガイドラインを記載する。
Tags
RNA Interference
American Cockroach
Gene Knockdown
Molecular Mechanisms
Development
Regeneration
Mating Behavior
DsRNA Injection
T7 Transcription
RNase A
RNase-free DNase
RNA Extraction
Microinjection
Article
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