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En trinvis metode til at detektere neutraliserende antistoffer mod AAV ved hjælp af et kolorimetrisk cellebaseret assay
JoVE Journal
Biology
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JoVE Journal Biology
A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay
DOI:

05:53 min

December 07, 2021

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Chapters

  • 00:05Introduction
  • 00:36Plating and Infecting HT1080 Cells
  • 02:00Day 3: Fixing and Adding Substrate to The Cells
  • 03:04Quantitation to Determine the Neutralizing Activity Using ImageJ
  • 03:48Results: Transduction Assay and Assessment of Neutralizing Antibodies (Nabs) Against AAV6 in Sheep Samples
  • 05:26Conclusion

Summary

Automatic Translation

En omfattende laboratorieprotokol og analysearbejdsgang er beskrevet for et hurtigt, omkostningseffektivt og ligetil kolorimetrisk cellebaseret assay til detektering af neutraliserende elementer mod AAV6.

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