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Journal
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Immunology and Infection
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狼瘡を起こしやすいMRL/lprマウスにおけるタンパク質尿、白血球の腎浸潤、およびタンパク質の腎沈着の解析
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
Please note that all translations are automatically generated.
Click here for the English version.
狼瘡を起こしやすいMRL/lprマウスにおけるタンパク質尿、白血球の腎浸潤、およびタンパク質の腎沈着の解析
DOI:
10.3791/63506-v
•
09:43 min
•
June 08, 2022
•
Xavier Cabana-Puig
,
Xin M. Luo
1
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine
,
Virginia Polytechnic Institute and State University
Chapters
00:05
Introduction
00:27
Proteinuria Test
01:17
Isolation of the Kidney Cells
04:51
Immunofluorescent Staining
07:39
Results: Characterizing Lupus Nephritis Based on Cell Infiltration and Protein Deposition in the Kidneys
09:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
本プロトコールは、マウスにおけるループス進行を追跡する方法を記載する。腎臓における細胞浸潤およびタンパク質沈着に基づいて狼瘡腎炎を特徴付けるために、2つの追加の手順が提示される。
Tags
Proteinuria
Renal Infiltration
Leukocytes
Renal Deposition
Proteins
Lupus-prone MRL/lpr Mice
Chronic Inflammation
Kidneys
Lupus
Urine Collection
Kidney Dissection
Cell Isolation
Percoll Gradient
Leukocyte Purification
Flow Cytometry
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