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Immunology and Infection
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Studying Inherited Immunity in a Caenorhabditis elegans Model of Microsporidia Infection
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Immunology and Infection
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JoVE Journal Immunology and Infection
Studying Inherited Immunity in a Caenorhabditis elegans Model of Microsporidia Infection

Studying Inherited Immunity in a Caenorhabditis elegans Model of Microsporidia Infection

DOI:
10.3791/63636-v

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09:24 min

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April 06, 2022

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, ,
1Department of Molecular Genetics,University of Toronto

Chapters

  • 00:04Introduction
  • 00:50Infection of C. elegans with N. Parisiito Yield Immune-Primed Offspring
  • 03:30Testing Inherited Immunity to N. Parisii in C. elegans and Analysis of DY96-Stained Worms to Assess Infection Status
  • 06:10Results: Study of Inherited Immunity in Genetically Tractable Worm Model
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

The infection of Caenorhabditis elegans by the microsporidian parasite Nematocida parisii enables the worms to produce offspring that are highly resistant to the same pathogen. This is an example of inherited immunity, a poorly understood epigenetic phenomenon. The present protocol describes the study of inherited immunity in a genetically tractable worm model.

Tags

Keywords: Caenorhabditis ElegansMicrosporidiaInherited ImmunityIntergenerational ImmunityInfectionSporesWormsOP50M9 MediaNematode Growth MediumCentrifugationMicroscopy

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