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Biology
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ヒト肺オルガノイドの確立と近位分化による成熟気道オルガノイドの生成
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Biology
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JoVE Journal
Biology
Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
Please note that all translations are automatically generated.
Click here for the English version.
ヒト肺オルガノイドの確立と近位分化による成熟気道オルガノイドの生成
DOI:
10.3791/63684-v
•
10:12 min
•
March 23, 2022
•
Cun Li
2
,
Man Chun Chiu
2
,
Yifei Yu
2
,
Xiaojuan Liu
2
,
Ding Xiao
2
,
Jingjing Huang
2
,
Zhixin Wan
2
,
Jie Zhou
2,3
1
Department of Microbiology, Li Ka Shing Faculty of Medicine
,
The University of Hong Kong
,
2
Centre for Virology, Vaccinology and Therapeutics
,
Hong Kong Science and Technology Park
,
3
State Key Laboratory of Emerging Infectious Diseases
,
The University of Hong Kong
Chapters
00:04
Introuduction
01:16
Derivation of Human Lung Organoid
03:25
Expansion of Human Lung Organoids
05:31
Proximal Differentiation to Generate Mature Airway Organoids
07:42
Results: Deriving Human Lung Organoids and Proximal Differentiation to Generate 3D and 2D Airway Organoids
09:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、初代肺組織からヒト肺オルガノイドを誘導し、肺オルガノイドを拡張し、近位分化を誘導して、ヒト気道上皮を忠実に表現鏡検査する3Dおよび2D気道オルガノイドを生成する方法を提示する。
Tags
Lung Organoids
Airway Epithelium
3D Culture
Cell Isolation
Collagenase Digestion
Basement Matrix
Expansion Medium
Proximal Differentiation
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