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Medicine
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分离肺内动脉和平滑肌细胞以研究血管反应
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Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses
Please note that all translations are automatically generated.
Click here for the English version.
分离肺内动脉和平滑肌细胞以研究血管反应
DOI:
10.3791/63686-v
•
07:56 min
•
June 08, 2022
•
Kittiwoot To-on
,
Usana Chatturong
,
Teerapap Panklai
,
Iyapa Palang
,
Anjaree Inchan
,
Sutthinee Wisutthathum
,
Tamkeen Urooj Paracha
,
Phapada Apaikawee
,
Krongkarn Chootip
1
Department of Physiology, Faculty of Medical Science and Center of Excellence for Innovation in Chemistry
,
Naresuan University
,
2
Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences
,
Naresuan University
,
3
Cosmetics and Natural Products Research Center, Faculty of Pharmaceutical Sciences
,
Naresuan University
,
4
Snyder Institute for Chronic Diseases, Airways Inflammation Research Group, Department of Physiology and Pharmacology
,
University of Calgary
Chapters
00:05
Introduction
01:26
Isolation of Intrapulmonary Artery (IPA)
02:15
Isolation of Vascular Smooth Muscle Cells (VSMCs)
03:18
Organ Bath Technique
04:45
Vasorelaxant Response to Plant Extract
05:21
Mechanism of Plant Extract-Induced Vasorelaxation via the Endothelium
05:54
Results: Mechanistic Basis of the Vasorelaxant Action of the Plant Extract on Isolated Rat IPA and VSMCs
07:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
动脉肺循环的血管反应可以使用肺内动脉 (IPA) 和血管平滑肌细胞 (VSMC) 进行探索。本研究详细描述了IPA的分离以及用于研究对生理刺激的反应的血管松弛的方案。
Tags
Keywords: Intrapulmonary Artery
Vascular Smooth Muscle Cells
Organ Bath Technique
Vascular Physiology
Pulmonary Hypertension
Papain
Collagenase
Cell Isolation
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