A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Physiology, Faculty of Medical Science and Center of Excellence for Innovation in Chemistry,Naresuan University, 2Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences,Naresuan University, 3Cosmetics and Natural Products Research Center, Faculty of Pharmaceutical Sciences,Naresuan University, 4Snyder Institute for Chronic Diseases, Airways Inflammation Research Group, Department of Physiology and Pharmacology,University of Calgary
Chapters
- 00:05Introduction
- 01:26Isolation of Intrapulmonary Artery (IPA)
- 02:15Isolation of Vascular Smooth Muscle Cells (VSMCs)
- 03:18Organ Bath Technique
- 04:45Vasorelaxant Response to Plant Extract
- 05:21Mechanism of Plant Extract-Induced Vasorelaxation via the Endothelium
- 05:54Results: Mechanistic Basis of the Vasorelaxant Action of the Plant Extract on Isolated Rat IPA and VSMCs
- 07:18Conclusion
Vaskulära svar på arteriell lungcirkulation kan utforskas med hjälp av intrapulmonell artär (IPA) och vaskulära glattmuskelceller (VSMCs). Den aktuella studien beskriver isoleringen av IPA i detalj och de protokoll som används för att undersöka vasorelaxation som svar på fysiologiska stimuli.
